{"id":987509621,"date":"2024-06-24T08:30:28","date_gmt":"2024-06-24T08:30:28","guid":{"rendered":"https:\/\/cidr.iisc.ac.in\/amit\/?p=987509621"},"modified":"2024-06-24T08:30:56","modified_gmt":"2024-06-24T08:30:56","slug":"transmission-electron-microscopic-tem-analysis-ofmtbnucleoids","status":"publish","type":"post","link":"https:\/\/cidr.iisc.ac.in\/amit\/2024\/06\/24\/transmission-electron-microscopic-tem-analysis-ofmtbnucleoids\/","title":{"rendered":"Transmission electron microscopic (TEM) analysis ofMtbnucleoids."},"content":{"rendered":"<p><strong>Transmission electron microscopic (TEM) analysis of<em>Mtb<\/em>nucleoids<\/strong>. TEM images of the longitudinal and transverse sections of: (<strong>A, B<\/strong>) wt\u00a0<em>Mtb<\/em>\u00a0from mid-log phase culture showing a well spread out nucleoid in the middle of the cell;\u00a0<strong>(C, D)<\/strong><em>whiB4-OE<\/em>\u00a0cells showing highly condensed nucleoid in the middle of the cell;\u00a0<strong>(E, F)<\/strong><em>Mtb<\/em>\u0394<em>whiB4<\/em>\u00a0showing unorganized and well spread out nucleoid throughout the cell;\u00a0<strong>(G, H)<\/strong><em>whiB4-comp<\/em>\u00a0showing nucleoid like wt\u00a0<em>Mtb<\/em>\u00a0(<strong>A, B<\/strong>).\u00a0<strong>(I, J)<\/strong><em>HU-OE<\/em>\u00a0showing highly condensed nucleoid, and\u00a0<strong>(K, L)<\/strong><em>TcrX-OE<\/em>\u00a0showing a well spread nucleoid. Arrowhead indicates nucleoid. For induction, 100\u2009ng\u2009ml-1\u00a0of Atc was used. As a control, the same amount of Atc was added to other strains. In each case, ~250\u2013300 independent cells were visualized and representative images are shown.<\/p>\n<p><a href=\"https:\/\/doi.org\/10.1016\/j.redox.2018.08.006\">https:\/\/doi.org\/10.1016\/j.redox.2018.08.006<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Transmission electron microscopic (TEM) analysis ofMtbnucleoids. TEM images of the longitudinal and transverse sections of: (A, B) wt\u00a0Mtb\u00a0from mid-log phase culture showing a well spread out nucleoid in the middle of the cell;\u00a0(C, D)whiB4-OE\u00a0cells showing highly condensed nucleoid in the middle of the cell;\u00a0(E, F)Mtb\u0394whiB4\u00a0showing unorganized and well spread out nucleoid throughout the cell;\u00a0(G, H)whiB4-comp\u00a0showing [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":""},"categories":[12],"tags":[],"_links":{"self":[{"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/posts\/987509621"}],"collection":[{"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/comments?post=987509621"}],"version-history":[{"count":1,"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/posts\/987509621\/revisions"}],"predecessor-version":[{"id":987509622,"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/posts\/987509621\/revisions\/987509622"}],"wp:attachment":[{"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/media?parent=987509621"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/categories?post=987509621"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/tags?post=987509621"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}