{"id":987509630,"date":"2024-06-24T08:43:42","date_gmt":"2024-06-24T08:43:42","guid":{"rendered":"https:\/\/cidr.iisc.ac.in\/amit\/?p=987509630"},"modified":"2024-06-24T08:46:20","modified_gmt":"2024-06-24T08:46:20","slug":"diagram-illustrating-the-principle-of-rogfp2-based-sensors","status":"publish","type":"post","link":"https:\/\/cidr.iisc.ac.in\/amit\/2024\/06\/24\/diagram-illustrating-the-principle-of-rogfp2-based-sensors\/","title":{"rendered":"\u00a0Diagram illustrating the principle of roGFP2 based sensors.\u00a0"},"content":{"rendered":"<p><img decoding=\"async\" loading=\"lazy\" class=\"alignnone wp-image-987509629 size-full\" src=\"https:\/\/cidr.iisc.ac.in\/amit\/wp-content\/uploads\/2024\/06\/research-image_0002_Vector-Smart-Object.jpg\" alt=\"\" width=\"883\" height=\"600\" srcset=\"https:\/\/cidr.iisc.ac.in\/amit\/wp-content\/uploads\/2024\/06\/research-image_0002_Vector-Smart-Object.jpg 883w, https:\/\/cidr.iisc.ac.in\/amit\/wp-content\/uploads\/2024\/06\/research-image_0002_Vector-Smart-Object-480x326.jpg 480w\" sizes=\"(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) 883px, 100vw\" \/><\/p>\n<p>(A) roGFP2 contains two Cys residues capable of forming an intramolecular disulfide bond in response to changes in intracellular\u00a0<em>E<\/em><em>GSH<\/em>. The redox equilibrium of roGFP2 with the GSH\/GSSG couple occurs through endogenous glutaredoxins and proceeds slowly. (B) Fusion of human Grx1 in Grx1-roGFP2 substantially improved specificity and rate of thiol-disulfide exchange between roGFP2 dithiol and 2GSH\/GSSG couple. (C) Replacing Grx1 with Mrx1 in Mrx1-roGFP2 ensured continuous equilibration between roGFP2 and 2MSH\/MSSM pair.<\/p>\n<p><a href=\"https:\/\/doi.org\/10.1371\/journal.ppat.1003902\">https:\/\/doi.org\/10.1371\/journal.ppat.1003902<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>(A) roGFP2 contains two Cys residues capable of forming an intramolecular disulfide bond in response to changes in intracellular\u00a0EGSH. The redox equilibrium of roGFP2 with the GSH\/GSSG couple occurs through endogenous glutaredoxins and proceeds slowly. (B) Fusion of human Grx1 in Grx1-roGFP2 substantially improved specificity and rate of thiol-disulfide exchange between roGFP2 dithiol and 2GSH\/GSSG [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":""},"categories":[12],"tags":[],"_links":{"self":[{"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/posts\/987509630"}],"collection":[{"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/comments?post=987509630"}],"version-history":[{"count":2,"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/posts\/987509630\/revisions"}],"predecessor-version":[{"id":987509632,"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/posts\/987509630\/revisions\/987509632"}],"wp:attachment":[{"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/media?parent=987509630"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/categories?post=987509630"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/cidr.iisc.ac.in\/amit\/wp-json\/wp\/v2\/tags?post=987509630"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}